Test genetyczny
do wykrywania
koronawirus (2019-nCoV)
certyfikat CE-IVD


System do zastosowań 
profesjonalnych metodą RT-PCR
Real-Time OneStep

OPIS PRODUKTU

It is screening kit to detect Novel Corona Virus as 2019-nCoV from isolated total RNA. Basic principal of this kit is Real-time PCR method, which is able to detect specific target gene into total RNA. One step RT-PCR contents are progressively apply for one tube RT(Reverse Transcription) reaction and PCR amplification. You can monitoring the nucleic acid amplification result based on real time condition through the amplification plot. The Control Template (2019-nCoV) is provide as positive control to assist the clinical sample data comparison analysis.

Features

- Simple & Rapid detection system: OneStep Multiplex RT-qPCR based detection
- HotStart PCR: high-specificity
- Reliable system: automatic PCR control (not Internal control)
- Easy-to-use master mix: just adding template and Primer/Probe Mix
- Positive control included (Plasmid)

Experimental



Contents

components SQD52-K020 SQD52-K100
2X OneStep qRT-PCR Buffer (2019-nCoV) 200 ul x 1 ea 1.0 ml x 1ea
OneStep qRT-PCR Enzyme mix (2019-nCoV) 40 ul x 1 ea 200 ul x 1 ea
Primer & Probe Mixture (2019-nCoV) 60 ul x 1 ea 300 ul x 1 ea
Control Template (2019-nCoV) 20 ul x 1 ea 100 ul x 1 ea
RNase Free Water 200 ul x 1 ea 1.0 ml x 1 ea

Specification

Detection target 2019-nCoV ( COVID-19 )
Target region Orf1a, N gene
Detection technology Real-Time OneStep RT-qPCR
Specimen type Nasopharyngeal swab, Oropharyngeal swab, Sputum
Compatible instruments* CFX96™ Real-Time PCR System (Bio-Rad)
ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems)
PCR running time ~ 120 mins

Types 5' Fluorophore 3' Quencher
N gene FAM BHQ1
orf1a VIC/JOE* BHQ1
PCR control Texas Red/Cal Fluor Red 610* BHQ2

*ABI 7500 / 7500 Fast: JOE, Texas Red │ Bio Rad CFX96™: VIC, Cal Fluor Red 610


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